Preparation of biological samples containing both metallic and organic structures for SEM

Authors

  • Fernando Vacilotto Gomes Faculdade de Odontologia da UFRGS
  • Carlos Eduardo Baraldi UFRGS
  • Deise Ponzoni Faculdade de Odontologia da UFRGS
  • Marília Gerhardt de Oliveira Hospital Cristo Redentor - Grupo Hospitalar Conceição
  • Edela Puricelli Faculdade de Odontologia da UFRGS

DOI:

https://doi.org/10.9771/cmbio.v12i2.7985

Keywords:

Dental Implants. Scanning Electron Microscopy. Methodological Study. Dentistry.

Abstract

Objective: results of preclinical studies depend on high-quality sample preparation, which enables proper handling of specimens for observation and analysis with the desired methods. The aim of this paper is to describe a step-by-step method for preparation of bone tissue specimens containing metallic implants for scanning electron microscopy (SEM). Methodology: eight rabbit bone specimens containing one osseointegrated implant each were fixated in 10% neutral buffered formalin, dehydrated, sectioned, and embedded

in thermosetting resin. The specimens were then sanded, polished, and metal-coated for SEM analysis. Results: the method achieved satisfactory specimen surface smoothness, containing no cracks or other artifacts, enabling morphological and chemical analysis by means of SEM and energy-dispersive X-ray spectroscopy (EDS). Conclusion: this method for preparation of animal tissue samples

containing both organic and metal components produced specimens amenable to SEM analysis with excellent image quality, enabling assessment of the bone–implant interface, measurement of bone–implant contact, and quantification of bone formation.

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Author Biographies

Fernando Vacilotto Gomes, Faculdade de Odontologia da UFRGS

Cirurgião dentista. Aluno de Pós-Graduação em Odontologia na UFRGS.

Carlos Eduardo Baraldi, UFRGS

Departamento de Cirurgia e Ortopedia Faculdade de Odontologia Universidade Federal do Rio Grande do Sul

Deise Ponzoni, Faculdade de Odontologia da UFRGS

Departamento de Cirurgia e Ortopedia Faculdade de Odontologia Universidade Federal do Rio Grande do Sul

Marília Gerhardt de Oliveira, Hospital Cristo Redentor - Grupo Hospitalar Conceição

Pesquisadora por Produtividade - CNPq

Hospital Cristo Redentor do Grupo Hospitalar Conceição – Ministério da Saúde 

Edela Puricelli, Faculdade de Odontologia da UFRGS

Profa Titular, Departamento de Cirurgia e Ortopedia Faculdade de Odontologia Universidade Federal do Rio Grande do Sul. Hospital de Clínicas de Porto Alegre

Published

2013-11-08

How to Cite

Gomes, F. V., Baraldi, C. E., Ponzoni, D., de Oliveira, M. G., & Puricelli, E. (2013). Preparation of biological samples containing both metallic and organic structures for SEM. Journal of Medical and Biological Sciences, 12(2), 219–223. https://doi.org/10.9771/cmbio.v12i2.7985

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