Proliferation of human bone marrow stem cells cultured on alfa-tricalcium phosphate cement and titanium discs

Autores

  • Marcel Fasolo de Paris
  • Carlos Eduardo Baraldi
  • Christian Viezzer
  • Denise Cantarelli Machado
  • Marília Gerhardt de Oliveira

DOI:

https://doi.org/10.9771/cmbio.v13i1.8992

Palavras-chave:

Stem cells. Calcium phosphates. Titanium.

Resumo

Objectives: to evaluate human bone marrow stem cells (HBMSC) cultured on α -tricalcium phosphate cement (α -TCP) and titanium (Ti) discs, as well as the osteoconductive properties of these biomaterials. Methods: discs of α -TCP and commercially pure Ti weremanufactured and placed on polyethylene plates. Cells were cultured according to the following: group I – control (no discs); group II – α-TCP discs; group III – Ti discs. Propidium iodide staining was used to assess cell proliferation. Cells cultures were analyzed at 3,7, 14 and 21 days. Results: Proliferation occurred in both biomaterials. At 3 days, greater cell proliferation was seen in group II than group III (p=0.01). Differences were not significant on the other time periods. Morphologic analysis showed circular morphology in group I, compatible with osteogenic cell lineage. Conclusions: both biomaterials were osteoconductive but not osteoinductive. This did not affect the initial stages of osteogenesis.

Downloads

Não há dados estatísticos.

Biografia do Autor

Marcel Fasolo de Paris

PhD, Universidade Federal do Rio Grande do Sul (UFRGS), Rua Ramiro Barcelos, 2492, Porto Alegre, RS, Brazil, 90035003. Phone +555133085194, Fax: +55133085010

Carlos Eduardo Baraldi

PhD, Universidade Federal do Rio Grande do Sul (UFRGS), Rua Ramiro Barcelos, 2492, Porto Alegre, RS, Brazil, 90035003. Phone +555133085194, Fax: +55133085010

Christian Viezzer

PhD, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Avenida Ipiranga, 6681, Porto Alegre, RS, Brazil, 90169900. Phone/Fax: +555133203538

Denise Cantarelli Machado

PhD, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Avenida Ipiranga, 6681, Porto Alegre, RS, Brazil, 90169900. Phone/Fax: +555133203538

Marília Gerhardt de Oliveira

PhD, Grupo Hospitalar Conceição (GHC), Ministério da Saúde, Rua Domingos Rubbo, 20B. Cristo Redentor, Porto Alegre, RS, Brazil, 91040000. Phone: +555133574100.

 

Downloads

Publicado

2014-06-18

Como Citar

de Paris, M. F., Baraldi, C. E., Viezzer, C., Machado, D. C., & Gerhardt de Oliveira, M. (2014). Proliferation of human bone marrow stem cells cultured on alfa-tricalcium phosphate cement and titanium discs. Revista De Ciências Médicas E Biológicas, 13(1), 7–12. https://doi.org/10.9771/cmbio.v13i1.8992

Artigos mais lidos pelo mesmo(s) autor(es)