Molecular Characterization in 3D Structure of MicroRNA Expressed in Leprosy

3D-Structural Characterization of MicroRNA Expressed in Leprosy

Autores/as

  • Luis Jesuino de Oliveira Andrade Universidade Estadual de Santa Cruz https://orcid.org/0000-0002-7714-0330
  • Humberto Barreto de Jesus Fundação Nacional de Saúde – Itabuna – Bahia – Brazil.
  • Luís Matos de Oliveira
  • Moema Farias de Oliveira
  • Laura Farias Caricchio Santana
  • Gabriela Correia Matos de Oliveira

DOI:

https://doi.org/10.9771/cmbio.v22i2.50816

Palabras clave:

Leprosy; MicroRNAs; Molecular structure.

Resumen

Introduction: Hansen's disease, or leprosy is caused by Mycobacterium leprae, is a major public health problem in developing countries, and affecting the skin and peripheral nerves. However, M. leprae can also affect bone tissue, mucous membranes, liver, eyes, and testicles, producing a variety of clinical phenotypes. MicroRNAs (miRNAs) have been expressed in the various clinical forms of leprosy and could potentially be used for its diagnosis.

Objective: In silico design of the molecular structure of miRNAs expressed in leprosy. Method: We performed a nucleotide sequence search of 17 miRNAs expressed in leprosy, designing in silico the molecular structure of the following miRNAs: miRNA-26a, miRNA-27a, miRNA-27b, miRNA-29c, miRNA-34c, miRNA-92a-1, miRNA-99a-2, miRNA-101-1, miRNA-101-2, miRNA-125b-1, miRNA-196b, miRNA-425-5p, miRNA-452, miRNA-455, miRNA-502, miRNA-539, and miRNA-660. We extracted the nucleotides were from the GenBank of National Center for Biotechnology Information genetic sequence database. We aligned the extracted sequences with the RNA Folding Form, and the three-dimensional molecular structure design was performed with the RNAComposer.

Results: We demonstrate the nucleotide sequences, and molecular structure projection of miRNAs expressed in leprosy, and produces a tutorial on the molecular model of the 17 miRNAs expressed in leprosy through in silico projection processing of their molecular structures.

Conclusion: We demonstrate in silico design of selected molecular structures of 17 miRNAs expressed in leprosy through computational biology.

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Biografía del autor/a

Humberto Barreto de Jesus, Fundação Nacional de Saúde – Itabuna – Bahia – Brazil.

Graduado em Medicina Universidade Federal da Bahia, Mestre em Saúde Coletiva Universidade Federal da Bahia, Médico Sanitarista Fundação Nacional de Saúde – Itabuna – Bahia – Brazil. https://orcid.org/0000-0002-0910-7168.

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Publicado

2023-09-13

Cómo citar

Andrade, L. J. de O., Jesus, H. B. de ., Oliveira, L. M. de ., Oliveira, M. F. de, Santana, L. F. C. ., & Oliveira, G. C. M. de . (2023). Molecular Characterization in 3D Structure of MicroRNA Expressed in Leprosy: 3D-Structural Characterization of MicroRNA Expressed in Leprosy. Revista De Ciências Médicas E Biológicas, 22(2), 188–196. https://doi.org/10.9771/cmbio.v22i2.50816

Número

Vol. 22 Núm. 2 (2023): Revista de Ciências Médicas e Biológicas

Sección

Artigos originais

Licencia

Derechos de autor 2023 Journal of Medical and Biological Sciences

Creative Commons License

Esta obra está bajo una licencia internacional Creative Commons Atribución 4.0.

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