Molecular Characterization in 3D Structure of MicroRNA Expressed in Leprosy

3D-Structural Characterization of MicroRNA Expressed in Leprosy

Authors

  • Luis Jesuino de Oliveira Andrade Universidade Estadual de Santa Cruz https://orcid.org/0000-0002-7714-0330
  • Humberto Barreto de Jesus Fundação Nacional de Saúde – Itabuna – Bahia – Brazil.
  • Luís Matos de Oliveira
  • Moema Farias de Oliveira
  • Laura Farias Caricchio Santana
  • Gabriela Correia Matos de Oliveira

DOI:

https://doi.org/10.9771/cmbio.v22i2.50816

Keywords:

Leprosy; MicroRNAs; Molecular structure.

Abstract

Introduction: Hansen's disease, or leprosy is caused by Mycobacterium leprae, is a major public health problem in developing countries, and affecting the skin and peripheral nerves. However, M. leprae can also affect bone tissue, mucous membranes, liver, eyes, and testicles, producing a variety of clinical phenotypes. MicroRNAs (miRNAs) have been expressed in the various clinical forms of leprosy and could potentially be used for its diagnosis.

Objective: In silico design of the molecular structure of miRNAs expressed in leprosy. Method: We performed a nucleotide sequence search of 17 miRNAs expressed in leprosy, designing in silico the molecular structure of the following miRNAs: miRNA-26a, miRNA-27a, miRNA-27b, miRNA-29c, miRNA-34c, miRNA-92a-1, miRNA-99a-2, miRNA-101-1, miRNA-101-2, miRNA-125b-1, miRNA-196b, miRNA-425-5p, miRNA-452, miRNA-455, miRNA-502, miRNA-539, and miRNA-660. We extracted the nucleotides were from the GenBank of National Center for Biotechnology Information genetic sequence database. We aligned the extracted sequences with the RNA Folding Form, and the three-dimensional molecular structure design was performed with the RNAComposer.

Results: We demonstrate the nucleotide sequences, and molecular structure projection of miRNAs expressed in leprosy, and produces a tutorial on the molecular model of the 17 miRNAs expressed in leprosy through in silico projection processing of their molecular structures.

Conclusion: We demonstrate in silico design of selected molecular structures of 17 miRNAs expressed in leprosy through computational biology.

Downloads

Download data is not yet available.

Author Biography

Humberto Barreto de Jesus, Fundação Nacional de Saúde – Itabuna – Bahia – Brazil.

Graduado em Medicina Universidade Federal da Bahia, Mestre em Saúde Coletiva Universidade Federal da Bahia, Médico Sanitarista Fundação Nacional de Saúde – Itabuna – Bahia – Brazil. https://orcid.org/0000-0002-0910-7168.

Downloads

Published

2023-09-13

How to Cite

Andrade, L. J. de O., Jesus, H. B. de ., Oliveira, L. M. de ., Oliveira, M. F. de, Santana, L. F. C. ., & Oliveira, G. C. M. de . (2023). Molecular Characterization in 3D Structure of MicroRNA Expressed in Leprosy: 3D-Structural Characterization of MicroRNA Expressed in Leprosy. Journal of Medical and Biological Sciences, 22(2), 188–196. https://doi.org/10.9771/cmbio.v22i2.50816

Issue

Vol. 22 No. 2 (2023): Revista de Ciências Médicas e Biológicas

Section

ORIGINAL ARTICLES

License

Copyright (c) 2023 Journal of Medical and Biological Sciences

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

The Journal of Medical and Biological Sciences reserves all copyrights of published works, including translations, allowing, however, their subsequent reproduction as transcription, with proper citation of source, through the Creative Commons license. The periodical has free and free access.