Early Detection of goats infected with Lentivirus Small Ruminant virus by ELISA assay

Auteurs

  • Silvia Inês Sardi Laboratório de Virologia, Instituto de Ciências da Saúde, Universidade Federal da Bahia.
  • Julianna Alves Torres Laboratório de Virologia, Instituto de Ciências da Saúde, Universidade Federal da Bahia
  • Camila Fonseca Lopes Brandão Laboratório de Virologia, Instituto de Ciências da Saúde, Universidade Federal da Bahia
  • Dellane Martins Tigre Laboratório de Virologia, Instituto de Ciências da Saúde, Universidade Federal da Bahia
  • Gubio Soares Campos Laboratório de Virologia, Instituto de Ciências da Saúde, Universidade Federal da Bahia

DOI :

https://doi.org/10.9771/cmbio.v11i1.5925

Mots-clés :

Lentiviruses, Ovine-Caprine. Enzyme-Linked Immunosorbent Assay. Diagnosis.

Résumé

Many established protocols are available to produce antigens for ELISA tests but expensive equipments, the purification methods or recombinant technologies are not always available to veterinary diagnostic laboratories. The aim of this work was to develop an alternative ELISA test to detect antibodies against Lentivirus Small Ruminant Virus (Caprine arthritis-encephalitis virus-CAEV) in goats, simple to elaborate with a low cost of production. The antigen was obtain from a whole cellular lysates of goat synovial membrane cells (GSMC) infected with CAEV and treated with SDS 0,1%. The whole-viral antigen was capable of detecting antibodies against several viral proteins including the p28 kDa (viral capsid), the p33 kDa, the p44 kDa (capsid precursors) and p97 kDa (transmembrane glycoprotein). The comparison with others assays as indirect immunofluorescence showed a high correlation with the ELISA results and the polimerasse chain reaction (PCR) demonstrated that most of the goat seropositives by ELISA, had proviral DNA in the milk cells. In conclusion, the viral antigen obtained from GSMC was simple to elaborate, low cost of production. The ELISA test was capable of detecting antibodies against CAEV, to detect early infection.

Téléchargements

Les données relatives au téléchargement ne sont pas encore disponibles.

Biographie de l'auteur

Silvia Inês Sardi, Laboratório de Virologia, Instituto de Ciências da Saúde, Universidade Federal da Bahia.

Possui graduação em Medicina Veterinaria - Universidad de Buenos Aires (1983) e doutorado em Virologia - Universidad de Buenos Aires (1996). Atualmente é professor adjunto 1-coordenador do laboratorio de virologia da Universidade Federal da Bahia. Atua na pos-graduação da Escola de Medicina Veterinaria, e no Instituto de Ciencias da Saude:Imunologia da UFBA. Tem experiência na área de Virologia, atuando na area das viroses de interesse veterinario e humano.

Téléchargements

Publiée

2012-09-24

Comment citer

Sardi, S. I., Torres, J. A., Brandão, C. F. L., Tigre, D. M., & Campos, G. S. (2012). Early Detection of goats infected with Lentivirus Small Ruminant virus by ELISA assay. Revista De Ciências Médicas E Biológicas, 11(1), 35–40. https://doi.org/10.9771/cmbio.v11i1.5925

Articles les plus lus par le même auteur ou la même autrice