Proliferation of human bone marrow stem cells cultured on alfa-tricalcium phosphate cement and titanium discs

Autores/as

  • Marcel Fasolo de Paris
  • Carlos Eduardo Baraldi
  • Christian Viezzer
  • Denise Cantarelli Machado
  • Marília Gerhardt de Oliveira

DOI:

https://doi.org/10.9771/cmbio.v13i1.8992

Palabras clave:

Stem cells. Calcium phosphates. Titanium.

Resumen

Objectives: to evaluate human bone marrow stem cells (HBMSC) cultured on α -tricalcium phosphate cement (α -TCP) and titanium (Ti) discs, as well as the osteoconductive properties of these biomaterials. Methods: discs of α -TCP and commercially pure Ti weremanufactured and placed on polyethylene plates. Cells were cultured according to the following: group I – control (no discs); group II – α-TCP discs; group III – Ti discs. Propidium iodide staining was used to assess cell proliferation. Cells cultures were analyzed at 3,7, 14 and 21 days. Results: Proliferation occurred in both biomaterials. At 3 days, greater cell proliferation was seen in group II than group III (p=0.01). Differences were not significant on the other time periods. Morphologic analysis showed circular morphology in group I, compatible with osteogenic cell lineage. Conclusions: both biomaterials were osteoconductive but not osteoinductive. This did not affect the initial stages of osteogenesis.

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Biografía del autor/a

Marcel Fasolo de Paris

PhD, Universidade Federal do Rio Grande do Sul (UFRGS), Rua Ramiro Barcelos, 2492, Porto Alegre, RS, Brazil, 90035003. Phone +555133085194, Fax: +55133085010

Carlos Eduardo Baraldi

PhD, Universidade Federal do Rio Grande do Sul (UFRGS), Rua Ramiro Barcelos, 2492, Porto Alegre, RS, Brazil, 90035003. Phone +555133085194, Fax: +55133085010

Christian Viezzer

PhD, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Avenida Ipiranga, 6681, Porto Alegre, RS, Brazil, 90169900. Phone/Fax: +555133203538

Denise Cantarelli Machado

PhD, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Avenida Ipiranga, 6681, Porto Alegre, RS, Brazil, 90169900. Phone/Fax: +555133203538

Marília Gerhardt de Oliveira

PhD, Grupo Hospitalar Conceição (GHC), Ministério da Saúde, Rua Domingos Rubbo, 20B. Cristo Redentor, Porto Alegre, RS, Brazil, 91040000. Phone: +555133574100.

 

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Publicado

2014-06-18

Cómo citar

de Paris, M. F., Baraldi, C. E., Viezzer, C., Machado, D. C., & Gerhardt de Oliveira, M. (2014). Proliferation of human bone marrow stem cells cultured on alfa-tricalcium phosphate cement and titanium discs. Revista De Ciências Médicas E Biológicas, 13(1), 7–12. https://doi.org/10.9771/cmbio.v13i1.8992

Número

Vol. 13 Núm. 1 (2014): REVISTA DE CIÊNCIAS MÉDICAS E BIOLÓGICAS

Sección

Artigos originais

Licencia

A Revista de Ciências Médicas e Biológicas reserva-se todos os direitos autorais dos trabalhos publicados, inclusive de tradução, permitindo, entretanto, a sua posterior reprodução como transcrição, com a devida citação de fonte. O periódico tem acesso livre e gratuito.