Detection of human rotavirus in children with diarrhea episodes in Salvador, Bahia, Brazil.

Authors

  • Gúbio Soares Campos Universidade Federal da Bahia
  • Sílvia Inês Sardi
  • Aline Santos Sampaio
  • Viviane Brandão Bandeira de Mello

DOI:

https://doi.org/10.9771/cmbio.v1i1.4042

Keywords:

Rotavírus humano, diagnóstico, eletroferotipos

Abstract

Acute infantile diarrhea is an important problem in public healthcare and Rotaviruses are responsible for about 20-40% of the cases of severe dehydrating diarrhea in children at 0-5 years old. In this work, we have analyzed diarrheic feces from children at 0-4 years old hospitalized between October 1998 and December 1999, in Salvador, Bahia, Brazil. In order to detect rotavirus, the samples were analyzed by ELISA (Test-Pack Abbot) and SDS-PAGE tests. The results demonstrated that from the 217 samples tested, 76 (35%) were positive, 127 (58,5 %) were negative and 14 (6,5%) diverged in both tests. The positive samples analyzed by SDS-PAGE test revealed the group A rotavirus and they were classified as short eletropherotypes (Subgroup I) and long eletropherotypes (Subgroup II).

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Published

2002-07-01

How to Cite

Campos, G. S., Sardi, S. I., Sampaio, A. S., & Mello, V. B. B. de. (2002). Detection of human rotavirus in children with diarrhea episodes in Salvador, Bahia, Brazil. Journal of Medical and Biological Sciences, 1(1), 7–11. https://doi.org/10.9771/cmbio.v1i1.4042