Blood glucose from Nile tilapia juveniles anesthetized with clove oil

Abstract

The aim of this study was to evaluate the efficacy of clove oil on juvenile’s Nile tilapia anesthesia and to determine the optimal concentration for short exposure and achieving rapid procedures, and also to monitor the blood glucose concentration during fish exposure to the anesthetic. The concentrations of 30; 60; 120; 180; 240; 300; 350 and 400mg/L of clove oil were tested during a ten minutes exposure and the last two ones were used to determine the lethal concentration. Blood samples were used to evaluate glucose levels variations before, during and after anesthesia. The concentration of 30mg/L was considered the ideal to clove oil to induce deep anesthesia during rapid procedures as biometrics. The exposure to a concentration of 400mg/L of clove oil for ten minutes was lethal to the juveniles of Nile tilapia. The blood glucose concentration of the fishes before anesthesia was about 60,0 ± 3,40mg/dL which was the lesser value registered on the experiment. The blood glucose concentrations of the individuals sampled immediately after deep anesthesia (IAD), immediately after recovery (IAR) and 30 and 60 minutes after recovery (30 and 60 AR) were about 85,8 ± 7,04, 90,8 ± 9,37, 124,2 ± 18,62 e 110,2 ± 12,08mg/dL, respectively.