Semen quality and concentration of soluble proteins in the seminal plasma of Alpine bucks

Authors

  • Leonardo Franco Martins Universidade Federal de Viçosa
  • Maria Cristina Baracat-Pereira Universidade Federal de Viçosa
  • José Domingos Guimarães Universidade Federal de Viçosa
  • Simone Eliza Facione Guimarães Universidade Federal de Viçosa
  • Rogério Oliveira Pinho Universidade Federal de Viçosa

Abstract

It was aimed to study the in vitro seminal quality analyzed by complementary tests and to compare them with physical, morphological and biochemical aspects of male goat semen of the Alpine breed. This experiment took place at the Federal University of Viçosa, situated at 20º45’ S latitude and 42º51’ W longitude, Southwest of Brazil. It was done during the summer months of January and February, and three adult male goats of the Alpine breed were used in intensive conditions. The semen was collected by artificial vagina method. In all semen samples (45 ejaculates), after the physical and morphological analysis, the hiposmotic test was done. In 24 ejaculates, it were done thermo-resistance test, and in 21 ejaculates it were determined the concentration of total soluble proteins in seminal plasma. The male goats presented difference in the semen physical and morphological aspects, in the hiposmotic test and thermo-resistance test, but they did not presented difference in total soluble proteins concentration in seminal plasma. Results of the slow thermo-resistance test and hiposmotic test were positively correlated (r = 0.60). It was concluded, according to our results, that the concentration of total soluble proteins in seminal plasma can not be used as a parameter to predict the seminal quality of Alpine bucks.

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Author Biographies

Leonardo Franco Martins, Universidade Federal de Viçosa

Doutorando no departamento de medicina veterinária da Universidade Federal de Viçosa, na área de reprodução animal

Maria Cristina Baracat-Pereira, Universidade Federal de Viçosa

Professora no departamento de bioquímica e coordenadora do laboratório de Proteômica e bioquímica de proteínas da Universidade Federal de Viçosa

José Domingos Guimarães, Universidade Federal de Viçosa

Professor no departamento de medicina veterinária e coordenador do laboratório de reprodução animal da Universidade Federal de Viçosa

Simone Eliza Facione Guimarães, Universidade Federal de Viçosa

Professora no departamento de zootecnia e coordenadora do laboratório de melhoramento animal da Universidade Federal de Viçosa

Rogério Oliveira Pinho, Universidade Federal de Viçosa

Doutorando no departamento de medicina veterinária da Universidade Federal de Viçosa, na área de reprodução animal

Published

2010-05-18

Issue

Section

Animal Reproduction