Agar-gel immunodiffusion and rapid slide agglutination on canine brucellosis diagnosis

Abstract

The rapid slide agglutination test (SAT) was compared to the agar gel immunodiffusion test (AGID) for the canine brucellosis diagnosis in sera submitted or not to the treatment with 2-Mercaptoetanol. Among the 1,072 sera dogs examined in the study, 202 were positive by AGID test (18.84%), 9 by AGID 2-ME test (0.84%), 19 in the race for SAT (1, 77%) and 9 in the race for SAT 2-ME (0.84%). The statistical evaluation of the results showed no significant difference among the tests of SAT, SAT 2-ME and AGID 2-ME, but differed significantly from AGID test.