Different concentration of the ethylene glycol in nuclear chromatin organization of the preantral ovarian follicles from bovine (Bos indicus).

Abstract

The cryopreservation of the ovarian preantral follicles could help the conservation of several domestic and wild animal species. The objective of this investigation was to verify the effect of the ethylene glycol in different concentrations in nuclear organization de ovarian preantral follicles. Ovaries had been gotten in slaughter house. A toxicity test was conducted with strips of ovarian cortex using ethylene glycol (10, 20 or 40%). Tissues analysis were run using classic techniques of histology. The analysis of the morphologic organization of the nuclear chromatin of primordial follicles showed significant difference (P < 0.05) between the control group (81,1%) and 10% (60,5%), 20% (19,3%) and 40% (9,5%) groups. In primary folículos, significant differences between the control group and 20% (18,7%) and 40% (9,5%) groups (P < 0.05), but not between group 10% (65,5%). These results indicate that the cryoprotectant ethylene-glycol takes the morphologic alterations in the nuclear chromatin of primordial and primary follicles, enclosed in bovine ovarian tissue.