GTPase Rab5 and Rab7 proteins are modulated by Corynebacteriumpseudotuberculosis in murine macrophages

Abstract

Introdution: Corynebacterium pseudotuberculosis is an intracellular facultative bacterium that affects macrophages, causingcaseous lymphadenitis in goats and sheep. This disease is characterized by the formation of granulomas and results in significant economic losses for farmers of goats and sheep around the world. Phagosome maturation is essential for the death of intracellular pathogens, involving the fusion of the phagosome with early and late endocytic organelles, a process partially regulated by GTPase Rab5 and Rab7 proteins, respectively. Methods: We evaluated the ability of an attenuated T1 strain and a wild-type C57 strain of C. pseudotuberculosisto alter Rab5 and Rab7 protein expression in peritoneal CBA mouse macrophages following in vitro infection.Results:Rab5 and Rab7 expression decreased in macrophages infected with the C57 strain compared to uninfected macrophages, while a significant reduction only in Rab7 expression was observed in cells infected with T1. Nonetheless, both Rab5 and Rab7 expression was higher in macrophages infected with T1 in comparison to cells infected with the wild-type C57strain.Conclusions:Our studies indicatea greater degree of modulation of the endosomal proteins directly involved in phagosome maturationbyCorynebacterium pseudotuberculosis wild type.