Methodology to study the polymorphism of alcohol dehydrogenase gene
DOI:
https://doi.org/10.9771/cmbio.v7i2.4445Keywords:
Alcohol dehydrogenase, Genetic polymorphism, Alcohol dependence.Abstract
The main enzimes responsible for the metabolism of ethanol are alcohol dehidrogenase (ADH) and aldehyde dehidrogenase (ALDH). These enzymes are present in many forms and are encoded by different genes. Some of these genes are polymorphic and the enzymes encoded by these genes may present different metabolic efficiency towards alcohol and acetic aldehyde. These variations has been shown to influence the quantity of alcohol intake and in the higher risk of alcohol abuse and dependence. In this work we describe a method that allows to study the polymorfism of one of the genes for the alcohol dehidrogenase enzyme, the gene ADH1C. The DNA was isolated from volunteers and the polymorfism of the ADH1C gene was determined by polymerase chain reaction (PCR). Our results confirm the feasibility of the technique described by us to study the polymorfism of the ADH1C gene.Downloads
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Published
2008-03-02
How to Cite
Rebello, A. S., & Carvalho, M. da G. da C. (2008). Methodology to study the polymorphism of alcohol dehydrogenase gene. Journal of Medical and Biological Sciences, 7(2), 163–168. https://doi.org/10.9771/cmbio.v7i2.4445
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ORIGINAL ARTICLES
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