Detection of bacterial samples on the aquatic ecosystems adjacent to Saquarema Lagoon – Rio de Janeiro
DOI:
https://doi.org/10.9771/cmbio.v14i2.13395Palavras-chave:
Gram negative Bacilli, Enterobacteriaceae, multidrug-resistance, aquatic environment, plasmid DNAResumo
Introduction: Saquarema Lagoon (RJ) has a high ecological and economic value owing to its multiple uses. The population’s constant growth increases the amount of sewage containing bacteria and antimicrobial drugs that are discharged to the environment. Objectives: to detect Gram negative bacilli able to colonize or infect humans and animals and determine their antimicrobial resistance profiles. Methodology:samples were collected in the city centre in April 2010 and at Jaconé (Lagoon’s most preserved site) in February 2011. The total and thermo tolerant coliforms were determined and the isolation of samples was made using agar media containing 32cg/ mL of cephalotin. All samples were tested for antimicrobial susceptibility (AST) and on 16 samples, plasmid DNA was extracted. Results: different Gram negative bacteria were detected, such as: Enterobacter spp, Citrobacter freundii, Klebsiella pneumoniae and Pseudomonas aeruginosa. The coliform results showed that the water quality is proper for Human recreation. AST results demonstrated the existence of bacteria resistant to antimicrobial drugs frequently used in the community. It was possible to detected high molecular weight plasmids and nine samples (56,25%) showed at least one plasmid DNA electrophoresis band. Conclusions: there were not detected resistant samples to antimicrobial drugs normally used in hospital settings, which may possibly refute the idea of a contamination by nosocomial and/or veterinary sewage discharge.Downloads
Não há dados estatísticos.
Downloads
Publicado
2015-02-18
Como Citar
Nogueira, B. A., Olivella, J. G. B., Gil, A. C., Meirelles-Pereira, F., Gonçalves, V. D., Andrade, A. F. B. de, Bello, A. R., & Pereira, J. A. A. (2015). Detection of bacterial samples on the aquatic ecosystems adjacent to Saquarema Lagoon – Rio de Janeiro. Revista De Ciências Médicas E Biológicas, 14(2), 147–152. https://doi.org/10.9771/cmbio.v14i2.13395
Edição
Seção
Artigos originais
Licença
A Revista de Ciências Médicas e Biológicas reserva-se todos os direitos autorais dos trabalhos publicados, inclusive de tradução, permitindo, entretanto, a sua posterior reprodução como transcrição, com a devida citação de fonte. O periódico tem acesso livre e gratuito.